Publicaciones científicas

Aptamers Against Live Targets: Is In Vivo SELEX Finally Coming to the Edge?

04-sep-2020 | Revista: Molecular Therapy-Nucleic Acids

Mayte Sola, Ashwathi Puravankara Menon, Beatriz Moreno, Daniel Meraviglia-Crivelli, Mario Martínez Soldevilla, Fernando Cartón-García, Fernando Pastor


Abstract

Targeted therapeutics underwent a revolution with the entry of monoclonal antibodies in the medical toolkit. Oligonucleotide aptamers form another family of target agents that have been lagging behind in reaching the clinical arena in spite of their potential clinical translation. Some of the reasons for this might be related to the challenge in identifying aptamers with optimal in vivo specificity, and the nature of their pharmacokinetics. Aptamers usually show exquisite specificity, but they are also molecules that display dynamic structures subject to changing environments.

Temperature, ion atmosphere, pH, and other variables are factors that could determine the affinity and specificity of aptamers. Thus, it is important to tune the aptamer selection process to the conditions in which you want your final aptamer to function; ideally, for in vivo applications, aptamers should be selected in an in vivo-like system or, ultimately, in a whole in vivo organism. In this review we recapitulate the implementations in systematic evolution of ligands by exponential enrichment (SELEX) to obtain aptamers with the best in vivo activity.

CITA DEL ARTÍCULO  Mol Ther Nucleic Acids. 2020 Sep 4;21:192-204. doi: 10.1016/j.omtn.2020.05.025. Epub 2020 May 23.

Nuestros autores

Silueta investigadora
Ashwathi Puravankara Menon
Investigadora del Programa de Terapias Moleculares del Cima Universidad de Navarra
Beatriz Moreno Bruna
Investigadora Adscrito a Proyecto Programa de Investigación de Terapias Moleculares
Silueta investigador
Daniel Hackbrett Meraviglia-Crivelli de Caso
Fernando Pastor Rodríguez