Publicaciones científicas
An atlas of cells in the human tonsil
Ramon Massoni-Badosa 1 , Sergio Aguilar-Fernández 2 , Juan C Nieto 2 , Paula Soler-Vila 3 , Marc Elosua-Bayes 2 , Domenica Marchese 2 , Marta Kulis 3 , Amaia Vilas-Zornoza 4 , Marco Matteo Bühler 5 , Sonal Rashmi 2 , Clara Alsinet 2 , Ginevra Caratù 2 , Catia Moutinho 2 , Sara Ruiz 2 , Patricia Lorden 2 , Giulia Lunazzi 2 , Dolors Colomer 6 , Gerard Frigola 7 , Will Blevins 2 , Lucia Romero-Rivero 3 , Víctor Jiménez-Martínez 3 , Anna Vidal 3 , Judith Mateos-Jaimez 3 , Alba Maiques-Diaz 3 , Sara Ovejero 8 , Jérôme Moreaux 9 , Sara Palomino 10 , David Gomez-Cabrero 11 , Xabier Agirre 4 , Marc A Weniger 12 , Hamish W King 13 , Lucy C Garner 14 , Federico Marini 15 , Francisco Javier Cervera-Paz 16 , Peter M Baptista 16 , Isabel Vilaseca 17 , Cecilia Rosales 3 , Silvia Ruiz-Gaspà 3 , Benjamin Talks 18 , Keval Sidhpura 19 , Anna Pascual-Reguant 20 , Anja E Hauser 20 , Muzlifah Haniffa 21 , Felipe Prosper 22 , Ralf Küppers 12 , Ivo Glynne Gut 23 , Elias Campo 6 , José Ignacio Martin-Subero 24 , Holger Heyn 25
Abstract
Palatine tonsils are secondary lymphoid organs (SLOs) representing the first line of immunological defense against inhaled or ingested pathogens.
We generated an atlas of the human tonsil composed of >556,000 cells profiled across five different data modalities, including single-cell transcriptome, epigenome, proteome, and immune repertoire sequencing, as well as spatial transcriptomics.
This census identified 121 cell types and states, defined developmental trajectories, and enabled an understanding of the functional units of the tonsil. Exemplarily, we stratified myeloid slan-like subtypes, established a BCL6 enhancer as locally active in follicle-associated T and B cells, and identified SIX5 as putative transcriptional regulator of plasma cell maturation.
Analyses of a validation cohort confirmed the presence, annotation, and markers of tonsillar cell types and provided evidence of age-related compositional shifts.
We demonstrate the value of this resource by annotating cells from B cell-derived mantle cell lymphomas, linking transcriptional heterogeneity to normal B cell differentiation states of the human tonsil.
CITA DEL ARTÍCULO Immunity. 2024 Jan 30:S1074-7613(24)00031-1. doi: 10.1016/j.immuni.2024.01.006
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