Simple and Practical Multigram Synthesis of d-Xylonate Using a Recombinant Xylose Dehydrogenase
Israel Sánchez-Moreno, Raúl Benito-Arenas, Pilar Montero-Calle, Carmen Hermida, Eduardo García-Junceda, Alfonso Fernández-Mayoralas
An efficient multienzyme system for the preparative synthesis of d-xylonate, a chemical with versatile industrial applications, is described. The multienzyme system is based on d-xylose oxidation catalyzed by the xylose dehydrogenase from Calulobacter crescentus and the use of catalytic amounts of NAD+. The cofactor is regenerated in situ by coupling the reduction of acetaldehyde into ethanol catalyzed by alcohol dehydrogenase from Clostridium kluyveri. Excellent conversions (>95%) were obtained in a process that allows easy product isolation by simple evaporation of the volatile buffer and byproducts.
CITATION ACS Omega. 2019 Jun 18;4(6):10593-10598. doi: 10.1021/acsomega.9b01090. eCollection 2019 Jun 30.