Cellular Metabolism Service
BIOCHEMICAL ANALYSIS AND CELLULAR METABOLISM PLATFORM
The Cellular Metabolism Service at Cima is oriented to study various aspects of cellular metabolism and bioenergetics.
We have the cutting-edge and exclusive technology offered by the Seahorse XFp extracellular flow analyzer (Agilent). This is the only technology that allows the functional study of cellular bioenergetics in real-time through the analysis of the main energy production pathways of the cell: mitochondrial respiration and glycolysis. This equipment offers the possibility of performing several metabolic assays in a fast and simple way, being of great interest in the study of numerous diseases and therapies in research areas such as cancer, immunology, obesity and diabetes, neurodegeneration, etc.
Our service offers the researcher multiple functional assays in adherent and non-adherent cells, as well as in mitochondria isolated from tissue or cell culture. In addition, it offers the possibility to ask for advice in the choice of the test, in the optimization of the seeding density and drug concentration, and for the interpretation of the results.
RESPONSIBLE:
Eva Santamaria: evasmaria@external.unav.es
Phone: +34 948 194 700 - Ext. 814019
DAY OF SERVICE:
Tuesday and Thursday.
Do you need our services?
If you are interested in knowing our rates and contracting our services, please contact us.
Our services
For researchers and companies
Experience and technological capacity to develop different assays for the analysis of multiple parameters of cell metabolism.
Advice to the researcher
We provide researchers with our expertise in the choice of the test, in the design and conduct of the experiment, as well as in the interpretation of the results of metabolic assays.
Research activity
We innovate in the implementation of new metabolic tests to respond to user demands.
Cutting-edge technology
Seahorse XFp Analyzer
Seahorse XF analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multiwell plate. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis. These measurements provide a system-level view of cellular metabolic function in cultured cells and ex vivo samples. XF analyzers perform compound addition and mixing, label-free analytical screening, and automatic real-time measurement of OCR and ECAR.
LSM 800 Confocal Scanning Laser Microscope
The Confocal microscope allows observing the plane that is located at the focus point, eliminating optically through a diaphragm or "pinhole", the light coming from other planes.
It has a motorized stage and is equipped with four diode laser lines 405, 488, 561, 640, and three high sensitivity detectors and Airyscan detector for SUPERRESOLUTION (1.7 times higher than conventional confocal).
Preferably used for studies of marker colocalization, three-dimensional study of samples and in certain materials allows imaging of the sample surface by reflection.
Brain, dentate gyrus. Immunofluorescence for GFAP (green), sox-2 (red) and TOPRO-3 (blue).
Scientific activity of the
Cellular Metabolism Service
Latest scientific publications
- Molecular mechanisms promoting long-term cytopenia after BCMA CAR-T therapy in Multiple Myeloma Jul 26, 2024 | Magazine: Blood Advances
- Microproteins encoded by noncanonical ORFs are a major source of tumor-specific antigens in a liver cancer patient meta-cohort Jul 10, 2024 | Magazine: Science Advances
- Immune dysfunction prior to and during vaccination in multiple myeloma: a case study based on COVID-19 Jul 10, 2024 | Magazine: Blood Cancer Journal
- Epigenetic-based differentiation therapy for Acute Myeloid Leukemia Jul 2, 2024 | Magazine: Nature Communication