- [TERAPIA GÉNICA Y REGULACIÓN DE LA EXPRESIÓN GÉNICA]
- [TERAPIA GÉNICA DE ENFERMEDADES HEPÁTICAS]
- [TERAPIA GÉNICA DE ENFERMEDADES CONGÉNITAS CON AFECTACIÓN NEUROLÓGICA]
Danio Rerio as Model Organism for Adenoviral Vector Evaluation
Paola Gulías, Jorge Guerra-Varela, Manuela Gonzalez-Aparicio, Ana Ricobaraza, Africa Vales, Gloria Gonzalez-Aseguinolaza, Rubén Hernandez-Alcoceba, Laura Sánchez
Viral vector use is wide-spread in the field of gene therapy, with new clinical trials starting every year for different human pathologies and a growing number of agents being approved by regulatory agencies. However, preclinical testing is long and expensive, especially during the early stages of development. Nowadays, the model organism par excellence is the mouse (Mus musculus), and there are few investigations in which alternative models are used. Here, we assess the possibility of using zebrafish (Danio rerio) as an in vivo model for adenoviral vectors.
We describe how E1/E3-deleted adenoviral vectors achieve efficient transduction when they are administered to zebrafish embryos via intracranial injection. In addition, helper-dependent (high-capacity) adenoviral vectors allow sustained transgene expression in this organism. Taking into account the wide repertoire of genetically modified zebrafish lines, the ethical aspects, and the affordability of this model, we conclude that zebrafish could be an efficient alternative for the early-stage preclinical evaluation of adenoviral vectors.
CITA DEL ARTÍCULO Genes (Basel). 2019 Dec 17;10(12):1053. doi: 10.3390/genes10121053.