Publicaciones científicas

Protocol for in vivo CRISPR screening using selective CRISPR antigen removal lentiviral vectors

01-feb-2023 | Revista: STAR Protocols

Sarah Kate Lane-Reticker 1, Emily A Kessler 1, Audrey J Muscato 1, Sarah Y Kim 1, John G Doench 1, Kathleen B Yates 2, Robert T Manguso 3, Juan Dubrot 4


Abstract

Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR technology in vivo. Here, we present a protocol for genome engineering using selective CRISPR antigen removal (SCAR) lentiviral vectors in the Renca mouse model. This protocol describes conducting an in vivo genetic screen with a sgRNA library and SCAR vectors that can be applied to different cell lines and contexts. For complete details on the use and execution of this protocol, please refer to Dubrot et al. (2021).1.

Keywords: CRISPR; Cancer; Immunology; Sequencing.

CITA DEL ARTÍCULO STAR Protoc. 2023 Feb 1;4(1):102082. doi: 10.1016/j.xpro.2023.102082. Online ahead of print.

Nuestros autores

Dr. Juan Dubrot Armendariz
Investigador | Investigador principal Programa de Investigación en Tumores Sólidos