Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells
Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Iglesias-Garcia O (2), Lo Nigro A (3), Prosper F (4), Aranguren XL (5).
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector.
Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay.
Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.