Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

15-may-2017 | Revista: Stem Cell Research

Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Iglesias-Garcia O (2), Lo Nigro A (3), Prosper F (4), Aranguren XL (5).

RESUMEN

We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector.

Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay.

Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.

CITA DEL ARTÍCULO Stem Cell Res. 2017 May;21:40-43. doi: 10.1016/j.scr.2017.03.020. Epub 2017 Mar 29.

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