Publicaciones científicas
- [MEDICINA REGENERATIVA]
- [INGENIERÍA BIOMÉDICA]
- [GENERACIÓN DE ÓRGANOS IN VIVO MEDIANTE CÉLULAS MADRE]
Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells
Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Iglesias-Garcia O (2), Lo Nigro A (3), Prosper F (4), Aranguren XL (5).
RESUMEN
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector.
Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay.
Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.
CITA DEL ARTÍCULO Stem Cell Res. 2017 May;21:40-43. doi: 10.1016/j.scr.2017.03.020. Epub 2017 Mar 29.
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Nuestros autores
![Giulia Coppiello. Programa Medicina Regenerativa. Cima](/.imaging/mte/cima/profesional_carrusel-80x80/dam/cima/imagenes/profesionales/programas-transversales/medicina-regenerativa/giulia-coppiello/jcr:content/giulia-coppiello1.jpg)
![Gloria Abizanda. Programa Medicina Regenerativa. Cima](/.imaging/mte/cima/profesional_carrusel-80x80/dam/cima/imagenes/profesionales/programas-transversales/medicina-regenerativa/gloria-abizanda-sarasa/jcr:content/gloria-abizanda-sarasa.jpg)
![Elena Iglesias. Programa Medicina Regenerativa. Cima](/.imaging/mte/cima/profesional_carrusel-80x80/dam/cima/imagenes/profesionales/programas-transversales/medicina-regenerativa/elena-iglesias-lopez/jcr:content/elena-iglesias-lopez.jpg)
![Olalla Iglesias. Programa Medicina Regenerativa. Cima](/.imaging/mte/cima/profesional_carrusel-80x80/dam/cima/imagenes/profesionales/programas-transversales/medicina-regenerativa/olalla-iglesias-garcia/jcr:content/Olalla-Iglesias-web2.jpg)