Scientific publications

An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration. Scientific Publication

Sep 29, 2023 | Magazine: NPJ Regenerative Medicine

Juan Antonio Romero-Torrecilla  1   2 , José María Lamo-Espinosa  3   4 , Purificación Ripalda-Cemboráin  1   2   3   4 , Tania López-Martínez  1   2   4 , Gloria Abizanda  1   2 , Luis Riera-Álvarez  3 , Sergio Ruiz de Galarreta-Moriones  5 , Asier López-Barberena  5 , Naiara Rodríguez-Flórez  5   6 , Reyes Elizalde  5 , Vineetha Jayawarna  7 , José Valdés-Fernández  1   2 , Miguel Echanove-González de Anleo  1   2 , Peter Childs  8 , Elena de Juan-Pardo  9 , Manuel Salmeron-Sanchez  7 , Felipe Prósper  1   2   4   10   11 , Emma Muiños-López  12   13   14   15 , Froilán Granero-Moltó  16   17   18   19


Abstract

During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum.

Poly (ethyl acrylate) coating of the PCL membrane allowed functionalization, mediated by fibronectin and low dose recombinant human BMP-2 (rhBMP-2) (10-25 μg/ml), resulting in efficient, sustained osteoinduction in vitro.

In vivo, rhBMP-2 functionalized mimetic periosteum demonstrated regenerative potential in the treatment of rat critical-size femoral defects with highly efficient healing and functional recovery (80%-93%). Mimetic periosteum has also proven to be efficient for cell delivery, as observed through the migration of transplanted periosteum-derived mesenchymal cells to the bone defect and their survival.

Ultimately, mimetic periosteum demonstrated its ability to deliver key stem cells and morphogens to an injured site, exposing a therapeutic and translational potential in vivo when combined with unprecedentedly low rhBMP-2 doses.

CITATION  NPJ Regen Med. 2023 Sep 29;8(1):54.  doi: 10.1038/s41536-023-00330-2

Our authors

Gloria Abizanda Sarasa
Dr. Froilán Granero Molto