Scientific publications
Autophagy-mediated control of ribosome homeostasis in oncogene-induced senescence
Aida Rodríguez López 1, Maria H Jørgensen 1, Jesper F Havelund 2, Frederic S Arendrup 3, Srinivasa Prasad Kolapalli 1, Thorbjørn M Nielsen 1, Eva Pais 1, Carsten Jörn Beese 1, Ahmad Abdul-Al 1, Anna Constance Vind 4, Jiri Bartek 5, Simon Bekker-Jensen 4, Marta Montes 3, Panagiotis Galanos 1, Nils Faergeman 2, Lotta Happonen 6, Lisa B Frankel 7
Abstract
Oncogene-induced senescence (OIS) is a persistent anti-proliferative response that acts as a barrier against malignant transformation. During OIS, cells undergo dynamic remodeling, which involves alterations in protein and organelle homeostasis through autophagy. Here, we show that ribosomes are selectively targeted for degradation by autophagy during OIS. By characterizing senescence-dependent alterations in the ribosomal interactome, we find that the deubiquitinase USP10 dissociates from the ribosome during the transition to OIS.
This release of USP10 leads to an enhanced ribosome ubiquitination, particularly of small subunit proteins, including lysine 275 on RPS2. Both reinforcement of the USP10-ribosome interaction and mutation of RPS2 K275 abrogate ribosomal delivery to lysosomes without affecting bulk autophagy. We show that the selective recruitment of ubiquitinated ribosomes to autophagosomes is mediated by the p62 receptor. While ribophagy is not required for the establishment of senescence per se, it contributes to senescence-related metabolome alterations and facilitates the senescence-associated secretory phenotype.
CITA DEL ARTÍCULO Cell Rep. 2023 Nov 28;42(11):113381. doi: 10.1016/j.celrep.2023.113381. Epub 2023 Nov 5.
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