Scientific publications

Deconvolution of the hematopoietic stem cell microenvironment reveals a high degree of specialization and conservation

Apr 8, 2022 | Magazine: iScience

Jin Ye  1 , Isabel A Calvo  2   3 , Itziar Cenzano  2 , Amaia Vilas  2   3 , Xabier Martinez-de-Morentin  4 , Miren Lasaga  4 , Diego Alignani  2   3 , Bruno Paiva  2   3 , Ana C Viñado  2   3 , Patxi San Martin-Uriz  2 , Juan P Romero  2 , Delia Quilez Agreda  5 , Marta Miñana Barrios  5 , Ignacio Sancho-González  5 , Gabriele Todisco  6   7 , Luca Malcovati  6 , Nuria Planell  4 , Borja Saez  2   3 , Jesper N Tegner  1   8   9   10 , Felipe Prosper  2   3   11 , David Gomez-Cabrero  1   4   8   12   10


Abstract

Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets.

As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively.

Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species.

Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.

CITATION  iScience. 2022 Apr 8;25(5):104225.  doi: 10.1016/j.isci.2022.104225.  eCollection 2022 May 20

Our authors

Dr. Isabel Calvo Arnedo
Research Collaborator Myeloid Pathology Research Group
Dr. Itziar Cenzano Armendáriz
Diego Alignani
Senior Research Technician Cytometry Platform
Ana Cristina Viñado Solanas
Dr. Patxi San Martín Uriz
Project Researcher Multiple Myeloma Research Group
Investigador postdoctoral del Grupo de Patología Mieloide del Cima Universidad de Navarra
Juan Pablo Romero Riojas
Dr. Borja Sáez Ochoa