Publicaciones científicas

Deconvolution of the hematopoietic stem cell microenvironment reveals a high degree of specialization and conservation

08-abr-2022 | Revista: iScience

Jin Ye  1 , Isabel A Calvo  2   3 , Itziar Cenzano  2 , Amaia Vilas  2   3 , Xabier Martinez-de-Morentin  4 , Miren Lasaga  4 , Diego Alignani  2   3 , Bruno Paiva  2   3 , Ana C Viñado  2   3 , Patxi San Martin-Uriz  2 , Juan P Romero  2 , Delia Quilez Agreda  5 , Marta Miñana Barrios  5 , Ignacio Sancho-González  5 , Gabriele Todisco  6   7 , Luca Malcovati  6 , Nuria Planell  4 , Borja Saez  2   3 , Jesper N Tegner  1   8   9   10 , Felipe Prosper  2   3   11 , David Gomez-Cabrero  1   4   8   12   10


Abstract

Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets.

As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively.

Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species.

Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.

CITA DEL ARTÍCULO  iScience. 2022 Apr 8;25(5):104225.  doi: 10.1016/j.isci.2022.104225.  eCollection 2022 May 20

Nuestros autores

Isabel Calvo Arnedo
Colaborador de Investigación Grupo de Investigación en Patología Mieloide
Itziar Cenzano Armendáriz
Diego Alignani
Técnico Superior de Investigación Plataforma de Citometría
Ana Cristina Viñado Solanas
Patxi San Martín Uriz
Investigador Adscrito a Proyecto Grupo de Investigación en Mieloma Múltiple
Investigador postdoctoral del Grupo de Patología Mieloide del Cima Universidad de Navarra
Juan Pablo Romero Riojas
Borja Sáez Ochoa