Scientific publications

In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis

Aug 14, 2023 | Magazine: Nature Genetics

David Lara-Astiaso, Ainhoa Goñi-Salaverri, Julen Mendieta-Esteban, Nisha Narayan, Cynthia Del Valle, Torsten Gross, George Giotopoulos, Tumas Beinortas, Mar Navarro-Alonso, Laura Pilar Aguado-Alvaro, Jon Zazpe, Francesco Marchese, Natalia Torrea, Isabel A. Calvo, Cecile K. Lopez, Diego Alignani, Aitziber Lopez, Borja Saez, Jake P. Taylor-King, Felipe Prosper, Nikolaus Fortelny & Brian J. P. Huntly 


Abstract
Cellular differentiation requires extensive alterations in chromatin structure and function, which is elicited by the coordinated action of chromatin and transcription factors. By contrast with transcription factors, the roles of chromatin factors in differentiation have not been systematically characterized. Here, we combine bulk ex vivo and single-cell in vivo CRISPR screens to characterize the role of chromatin factor families in hematopoiesis. We uncover marked lineage specificities for 142 chromatin factors, revealing functional diversity among related chromatin factors (i.e. barrier-to-autointegration factor subcomplexes) as well as shared roles for unrelated repressive complexes that restrain excessive myeloid differentiation. Using epigenetic profiling, we identify functional interactions between lineage-determining transcription factors and several chromatin factors that explain their lineage dependencies. Studying chromatin factor functions in leukemia, we show that leukemia cells engage homeostatic chromatin factor functions to block differentiation, generating specific chromatin factor–transcription factor interactions that might be therapeutically targeted. Together, our work elucidates the lineage-determining properties of chromatin factors across normal and malignant hematopoiesis.

CITA DEL ARTÍCULO Nat Genet. 2023 Sep;55(9):1542-1554. doi: 10.1038/s41588-023-01471-2. Epub 2023 Aug 14.

Our authors

Mar Navarro Alonso
Laboratory technician Hemato-Oncology Research Program
Laura Aguado Álvaro
Jon Zazpe Estanislao
Laboratory technician Genomics Unit Platform
Dr. Francesco Paolo Marchese
Dr. Isabel Calvo Arnedo
Research Collaborator Hemato-Oncology Research Program
Diego Alignani
Laboratory technician Cytometry Platform
Aitziber López López
Laboratory technician Cytometry Platform