Services to researchers and companies

Genomics Platform

Advanced sequencing techniques

Undergoes rigorous quality controls to favor the biological result corresponding to each application or experiment

Protocols for RNA-seq: application usage recommendation and benefits
3'UTR RNAseq

Global gene expression analysis of polyA+ RNA. Only recommended for low/ultra-low input samples.

  • Sensitivity and reliability for low input material.
  • Some level of RNA degradation can be tolerated.
  • Use of unique molecular identifiers (UMI).
  • Low sequencing costs.
Full-length RNA-seq

Whole transcriptome analysis of polyA+ RNA. Only recommended for low/ultra-low input samples.

  • Sensitivity and reliability for low input material.
  • Uniform coverage and high confidence feature discovery (e.g. alternative transcripts, gene fusions, allele-specific expression, splicing variants).
Stranded Full-length RNAseq mRNA prep

Whole transcriptome analysis of polyA+ RNA with strand orientation information.

  • Analysis of coding and non-coding polyA+ RNAs with strand orientation measurement.
  • Uniform coverage and high-confidence feature discovery (e.g. alternative transcripts, gene fusions, allele-specific expression, splicing variants).
Stranded Full-length RNAseq total prep

Whole transcriptome analysis of total RNA with strand orientation information.

  • Sensitivity and reliability for low input material.
  • Some level of RNA degradation can be tolerated (e.g. FFPE samples).
  • Analysis of coding and non-coding RNAs with measurement of strand orientation.
  • Uniform coverage and high confidence feature discovery (e.g. alternative transcripts, gene fusions, allele-specific expression, splicing variants).
Protocols for DNA-seq: recommended application usage
Whole Exome Sequencing (WES)

Detection of genetic variants related to disease or phenotype in the coding region of the human genome.

  • Depth: A standard depth of 30X, 50X or 100X is offered but can be increased or decreased according to user preference.
Whole Genome Sequencing (WGS)

Detection of genetic variants related to disease or phenotype in coding and non-coding regions.

  • Depth: a standard depth of 30X or 40X (human and mouse genome) is offered but can be increased or decreased according to user preference.
     

Conversión de ADN obtenido desde una amplia gama de procedimientos (por ejemplo, ChIP-type procedures, amplicons, aptamer library, PCR products) en librerías de alta calidad para NGS.

Examples of accepted library types

The Genomics platform allows sequencing libraries of any type (compatible with Illumina platforms) prepared and quantified by the user. In this case, the platform cannot guarantee the quality or quantity of the final data.

Examples of accepted library types:

  • scRNAseq
  • MARSseq
  • Exomes
  • ChIP-seq
  • ATAC-seq

Need help?

If you have questions or doubts about your samples, please contact us.

Technological service innovation

In the Genomics Platform, we are currently in the last phase of developing new techniques that are more sensitive and complementary to the ones we offer.

To find out if the Cima Genomics Unit offers the library preparation or sequencing technology you need, please contact us.

How to submit a paper to the Genomics Platform?

STEP 1: Quotation and inquiries

To request a quote, discuss the project, questions, or more information about our services, you can contact us by sending an email to ngs_cima@unav.es.

STEP 2: Order

Fill in the order form and sample datasheet. Send the documents to ngs_cima@unav.es.

STEP 3: Sample reception

Deliver the samples to the laboratory 1.41 (CIMA Building), between 12:00-13:00 and 16:00-17:00 hours.

  • RNA: Normalize the RNA concentration of all samples to 10 ng/uL in a volume of 30 uL of 10 mM TRIS buffer pH 7.5 or TE buffer.
  • DNA: Normalize the DNA concentration of all samples to 50 ng/uL in a volume of 20 uL of 10 mM TRIS buffer pH 8 or TE buffer.

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  • If you wish the sequencing data to be analyzed by the Cima Bioinformatics Platform, you should also contact Mikel Hernáez.