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Protocols for RNA-seq: application usage recommendation and benefits

Stranded Full-length RNAseq mRNA prep

Whole transcriptome analysis of polyA+ RNA with strand orientation information.

• Analysis of coding and non-coding polyA+ RNAs with measurement of strand orientation.
• Uniform coverage and high-confidence feature discovery (e.g. alternative transcripts, gene fusions, allele-specific expression, splicing variants).

Stranded Full-length RNAseq total prep with ribo-zero plus

Whole transcriptome analysis of total RNA with strand orientation information.

• Sensitivity and reliability for low input material.
• Some level of RNA degradation can be tolerated (e.g. FFPE samples).
• Analysis of coding and non-coding RNAs with measurement of strand orientation.
• Uniform coverage and high confidence feature discovery (e.g. alternative transcripts, gene fusions, allele-specific expression, splicing variants).
• Translated with DeepL.com (free version)

Stranded Full-length RNAseq total prep with ribo-depletion (low/ultra-low input)

Whole transcriptome analysis of total RNA with strand orientation information.

• Sensitivity and reliability for low/ultra-low input material (250 pg - 10ng, >1500 cells).
• Use of unique molecular identifiers (UMI).
• Some level of RNA degradation can be tolerated.
• Analysis of coding and non-coding RNAs with measurement of strand orientation.
• Uniform coverage and high-confidence feature discovery (e.g. alternative transcripts, gene fusions, allele-specific expression, splicing variants).

Small RNAseq

Small RNA transcriptome analysis (15-150 nt)

• Small RNA analysis (miRNAs, siRNAs, piRNAs, snoRNAs).
• Sensitivity and reliability for low input material (total RNA or enriched small RNA, 1 ng-2 μg).