Publicaciones científicas
Ultrasensitive detection of circulating multiple myeloma cells by next-generation flow after immunomagnetic enrichment
Marta Lasa 1, Carmen Gonzalez 1, Laura Notarfranchi 1, Anastasiia Zherniakova 1, Diego Alignani 1, Leire Burgos 1, Maria José Calasanz 1, Paula Rodriguez-Otero 1, Jose J Perez 2, Clara Gomez 3, Veronica Gonzalez-Calle 2, Felipe de Arriba 4, Luis Palomera Bernal 5, Miguel Angel Alvarez Rivas 6, Esther Clavero Sanchez 7, Enrique M Ocio 8, Ana Pilar Gonzalez-Rodriguez 9, Sunil Lakhwani 10, Angela Ibañez 11, Albert Oriol 12, Anna Sureda 13, Laura Rosiñol 14, Christiane Siewert 15, Alberto Orfao 16, Juan-Jose Lahuerta 17, Joan Bladé 14, Maria-Victoria Mateos 2, Jesús F San-Miguel 1, Maria-Teresa Cedena 17, Joaquin Martinez-Lopez 17, Noemi Puig 2, Bruno Paiva 1
Abstract
The continuous improvement in progression-free survival (PFS) of multiple myeloma (MM) patients raises interest in evaluating peripheral residual disease (PRD) towards more frequent readouts of tumor kinetics while preserving quality of life. Here we present BloodFlow, a new method that combines immunomagnetic enrichment of CD138+ circulating plasma cells in peripheral blood (PB) with next-generation flow (NGF), for the detection of PRD below the 2x10-6 NGF threshold. BloodFlow detected PRD in 55/644 (8.5%) PB samples collected from 295 MM patients. Of note, 29/55 (52.7%) PB samples were positive using BloodFlow and negative by NGF. The lowest level of PRD detected by BloodFlow was 6x10-8. Considering patients' minimal residual disease (MRD) status in bone marrow as the reference, BloodFlow showed positive and negative predictive values of 95.1% and 76.6%. Presence of PRD during maintenance or observation predicted dismal progression-free and overall survival (2-year rates of 0% and 62%). BloodFlow surpassed NGF in PB and retained independent prognostic value for PFS in multivariate analysis including transplant-eligibility, the Revised International Staging System, complete remission and MRD status in bone marrow. BloodFlow is the first flow cytometry method that detects tumor cells below the 10-6 threshold, which resulted in improved minimally-invasive monitoring of MM patients.
CITA DEL ARTÍCULO Blood. 2025 May 22:blood.2025029234. doi: 10.1182/blood.2025029234. Online ahead of print.

Nuestros autores


