Scientific publications
T Cell Migration from Inflamed Skin to Draining Lymph Nodes Requires Intralymphatic Crawling Supported by ICAM-1/LFA-1 Interactions
Alvaro Teijeira 1 , Morgan C Hunter 2 , Erica Russo 2 , Steven T Proulx 2 , Thomas Frei 2 , Gudrun F Debes 3 , Marc Coles 4 , Ignacio Melero 5 , Michael Detmar 2 , Ana Rouzaut 5 , Cornelia Halin 6
Abstract
T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood.
Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels.
Intralymphatic T cell number and motility were increased during contact-hypersensitivity-induced inflammation and dependent on ICAM-1/LFA-1 interactions. In vitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors in skin explants.
In vivo, T cell migration to draining lymph nodes was significantly reduced upon ICAM-1 or LFA-1 blockade.
Our findings indicate that T cell migration through LVs occurs in distinct steps and reveal a key role for ICAM-1/LFA-1 interactions in this process.
CITA DEL ARTÍCULO Cell Rep. 2017 Jan 24;18(4):857-865. doi: 10.1016/j.celrep.2016.12.078.
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