Scientific publications

T Cell Migration from Inflamed Skin to Draining Lymph Nodes Requires Intralymphatic Crawling Supported by ICAM-1/LFA-1 Interactions

Jan 24, 2017 | Magazine: Cell Reports

Alvaro Teijeira  1 , Morgan C Hunter  2 , Erica Russo  2 , Steven T Proulx  2 , Thomas Frei  2 , Gudrun F Debes  3 , Marc Coles  4 , Ignacio Melero  5 , Michael Detmar  2 , Ana Rouzaut  5 , Cornelia Halin  6


T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood.

Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels.

Intralymphatic T cell number and motility were increased during contact-hypersensitivity-induced inflammation and dependent on ICAM-1/LFA-1 interactions. In vitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors in skin explants.

In vivo, T cell migration to draining lymph nodes was significantly reduced upon ICAM-1 or LFA-1 blockade.

Our findings indicate that T cell migration through LVs occurs in distinct steps and reveal a key role for ICAM-1/LFA-1 interactions in this process.

CITA DEL ARTÍCULO  Cell Rep. 2017 Jan 24;18(4):857-865. doi: 10.1016/j.celrep.2016.12.078.