Publicaciones científicas

Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma

01-ene-2022 | Revista: Disease Models & Mechanisms

Karmele Valencia  1   2   3   4 , Cristina Sainz  1   3 , Cristina Bértolo  1   3 , Gabriel de Biurrun  5 , Jackeline Agorreta  1   6 , Arantza Azpilikueta  7 , Marta J Larrayoz  1   8   2   3 , Graziella Bosco  9 , Carolina Zandueta  1   2 , Miriam Redrado  1   3 , Esther Redín  1   8   2   3 , Francisco Exposito  1   8   2   3 , Diego Serrano  1   8   3 , Mirari Echepare  1   8   3 , Daniel Ajona  1   2   3   4 , Ignacio Melero  2   3   7   10 , Ruben Pio  1   2   3   4 , Roman Thomas  9   11   12 , Alfonso Calvo  1   8   2   3 , Luis M Montuenga  1   8   2   3


Abstract

There is a paucity of adequate mouse models and cell lines available to study lung squamous cell carcinoma (LUSC).

We have generated and characterized two models of phenotypically different transplantable LUSC cell lines (UN-SCC679 and UN-SCC680) derived from an N-nitroso-tris-chloroethylurea (NTCU) chemically-induced mouse model in A/J mice. Furthermore, we genetically characterized and compared both LUSC cell lines by performing whole exome and RNA sequencing.

These experiments revealed similar genetic and transcriptomic patterns that may correspond to the classical LUSC human subtype. In addition, we compared the immune landscape generated by both tumor cells lines in vivo and assessed their response to immune checkpoint inhibition.

The differences between the two cell lines are a good model for the remarkable heterogeneity of human squamous cell carcinoma. Study of the metastatic potential of these models revealed that both cell lines represent the human LUSC organotropism to the brain, bones, liver and adrenal glands.

In summary, we have generated a very valuable cell line tools for LUSC research that recapitulates the complexity of the human disease.

CITA DE ARTÍCULO  Dis Model Mech. 2022 Jan 1;15(1):dmm049137.  doi: 10.1242/dmm.049137.  Epub 2022 Jan 31.

Nuestros autores

Alfonso Calvo González
Investigador | Investigador principal Programa de Investigación en Tumores Sólidos
Karmele Valencia Leoz
Colaborador de Investigación Programa de Investigación en Tumores Sólidos
Cristina Sainz Zubieta
Arantza Azpilicueta Lusarreta
Marta Larráyoz Ilundáin
Carolina Zandueta Pascual
Miriam Redrado Jordán
Esther Redín Resano
Francisco Expósito Rincón
Diego Serrano Tejero
Investigador Adscrito a Proyecto Programa de Investigación en Tumores Sólidos
Daniel Ajona Martínez-Polo